# Production of functional recombinant antibodies in Dictyostelium discoideum

## TL;DR

This paper shows that the organism Dictyostelium discoideum can produce functional antibodies, which could be useful for research and medicine.

## Contribution

The first demonstration of functional recombinant antibody production in Dictyostelium discoideum.

## Key findings

- Three recombinant antibodies were successfully produced and secreted by D. discoideum cells.
- Proteolytic cleavage at the linker peptide was suppressed by its removal.
- All three antibodies specifically recognized their target antigens in immunofluorescence analysis.

## Abstract

Recombinant antibodies are essential reagents for diagnostics, research, and therapy. Numerous production methods have been developed, each of them with its strengths and weaknesses. In this study we evaluated the ability of Dictyostelium discoideum cells to produce and secrete functional antibodies.

Three recombinant antibodies targeting tubulin, CISD1 or CD8β proteins, respectively, were successfully produced and secreted by D. discoideum cells. Electrophoretic analysis of these antibodies revealed a degradation product, resulting from proteolytic cleavage at the linker peptide connecting the scFv portion to the Fc fragment. Removal of this linker suppressed the proteolytic cleavage. Finally, immunofluorescence analysis confirmed that all three antibodies recognized their target antigen in a specific manner. This study represents the first demonstration that functional recombinant antibodies can be produced in D. discoideum cells.

The online version contains supplementary material available at 10.1186/s13104-025-07314-z.