# Single-cell analysis reveals significant transcriptomic alterations in preclinical Crohn’s disease

## TL;DR

This study uses single-cell RNA sequencing to identify early immune changes in preclinical Crohn’s disease patients.

## Contribution

The study explores transcriptomic alterations during the preclinical phase of Crohn’s disease, revealing early immune disturbances.

## Key findings

- Significant transcriptomic alterations were observed in preclinical Crohn’s disease patients.
- The study identified immune-related gene expression changes before clinical diagnosis.

## Abstract

Despite the increasing incidence of Crohn’s disease (CD), its early immune disturbances have not all been described yet. We analysed single-cell RNASeq data from peripheral blood mononuclear cells of patients with incidentally-diagnosed CD, and compared their gene expression profile to healthy individuals. The main aim of our study was to perform an exploratory analysis of how the subclinical inflammatory process is modifying the individual’s immunologic environment while the patient is still in the preclinical period.