# Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae)

## TL;DR

This paper presents a high-quality chromosome-level genome assembly for the lemon sole, a commercially important fish species.

## Contribution

The study provides the first chromosome-level genome assembly for Microstomus kitt using PacBio HiFi sequencing.

## Key findings

- The assembly has a contig N50 of 17.2 Mbp and scaffold N50 of 27.2 Mbp.
- The genome comprises 24 chromosome-length scaffolds with 99.7% complete BUSCO genes.

## Abstract

The lemon sole (Microstomus kitt) is a culinary fish from the family of righteye flounders (Pleuronectidae), inhabiting sandy, shallow offshore grounds of the North Sea, western Baltic Sea, English Channel, Great Britain and Ireland, Bay of Biscay, and coastal waters of Norway.

Here, we present a chromosome-level genome assembly of the lemon sole. We applied PacBio HiFi sequencing on the PacBio Revio system to generate a highly complete and contiguous reference genome.

The resulting assembly has a contig N50 of 17.2 Mbp and a scaffold N50 of 27.2 Mbp. The total assembly length is 628 Mbp, comprising 24 chromosome-length scaffolds. The identification of 99.7% complete BUSCO genes indicates a high level of assembly completeness.

The chromosome-level genome assembly of the lemon sole provides a high-quality reference genome for future population-level genomic analyses of this commercially valuable, edible fish.