# The orobasal organ (of Ackerknecht) is present in prenatal mice

## TL;DR

This study confirms the presence of the orobasal organ in prenatal mice, which may help understand its role in oral development and cyst formation.

## Contribution

The study demonstrates the existence of the orobasal organ in mouse embryos, resolving prior uncertainty.

## Key findings

- The orobasal organ appears in mice between prenatal days E15 and E17, with 90.5% prevalence in E17 embryos.
- The organ is present in both male and female embryos and has specific average dimensions at E17.
- This finding provides a basis for further research on the organ's development and function in a common model organism.

## Abstract

In 1912, the veterinary anatomist Eberhard Ackerknecht described morphologically highly variable epithelial invaginations behind the medial mandibular incisors. This orobasal organ (of Ackerknecht) is present in different mammalian species including humans, but its presence in mice was under debate in literature. While the function of the orobasal organ is still unknown, it might play a role in the development of cysts of the oral floor.

H&E-stained histological serial slides of the developing oral floor of C57BL/6J mice embryos were investigated (n = 40).

The orobasal organ was present in mice and developed between prenatal days E15 and E17 (prevalence in E15 embryos: 0%, prevalence in E17 embryos: 90.5%). The organ was present both in male and female embryos. In E17, the organ had an average size of 68.75 (±41.1) μm x 58.75 (±8.5) μm x 345 (±28.3) μm (length x depth x width).

While the existence of an orobasal organ was already shown for pre- and postnatal rats, there was only one publication dealing with the orobasal organ in mice. In this study, adult mice were investigated and no orobasal organ was found. Here, we demonstrate the existence of an orobasal organ in mice, at least in embryos. The presence of the orobasal organ in a common model organism will help to investigate its pre- and postnatal development, as well as possible physiological functions of this structure.