# Effect of ethylenediamine tetraacetic acid (EDTA) and maleic acid as root-end conditioners on the sealing ability of ProRoot mineral trioxide aggregate (MTA) and Biodentine as root-end filling materials: An in vitro study

**Authors:** Sumaiya Zafar, Yawar Ali Abidi, Samira Adnan, Maham Muneeb Lone, Farah Afroz Khan, Isma Sajjad

PMC · DOI: 10.34172/joddd.025.41232 · Journal of Dental Research, Dental Clinics, Dental Prospects · 2025-03-31

## TL;DR

This study compared how well MTA and Biodentine seal root-end cavities after using EDTA or maleic acid, finding Biodentine with maleic acid to be more effective.

## Contribution

The study evaluates the effect of EDTA and maleic acid on the sealing ability of MTA and Biodentine in root-end surgeries.

## Key findings

- Biodentine with maleic acid showed the least dye penetration, indicating better sealing.
- MTA with EDTA had the highest dye penetration, suggesting poorer sealing.
- Biodentine can be a viable alternative to MTA in root-end surgeries.

## Abstract

Apicoectomy is often required to treat a non-healing endodontic lesion. Materials used during this procedure, such as root-end conditioners and retrograde filling materials, can significantly affect the success. Mineral trioxide aggregate (MTA) has been used as a root-end filling material for a long time; however, success has also been reported with some novel materials such as Biodentine. The present study compared the sealing ability of MTA and Biodentine in root-end cavities following apicoectomy after using EDTA and maleic acid as root-end conditioners.

The apical one-third of 60 extracted teeth were resected, followed by root-end preparation of 3 mm. The samples were divided into 4 groups of 15 each. The roots in groups 1 and 2 were conditioned with EDTA and maleic acid in groups 3 and 4. Retrograde cavities in groups 1 and 3 were filled with MTA and in groups 2 and 4 with Biodentine. Microleakage was checked using the dye penetration method. One-way ANOVA and post hoc Tukey tests were used for statistical analyses.

Group 1 showed the highest (2.94±0.112), while group 4 had the least dye penetration (2.55±0.213).

It can be concluded that Biodentine showed better sealing ability after using maleic acid than MTA and can be used as an alternative to MTA in root-end surgeries.

## Linked entities

- **Chemicals:** ethylenediamine tetraacetic acid (PubChem CID 6049), EDTA (PubChem CID 6049), maleic acid (PubChem CID 444266), MTA (PubChem CID 439176), Biodentine (PubChem CID 25523)

## Full-text entities

- **Diseases:** endodontic lesion (MESH:D011671)
- **Chemicals:** EDTA (MESH:D004492), Biodentine (MESH:C506393), maleic acid (MESH:C030272), MTA (MESH:C086631)

## Full text

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## References

23 references — full list in the complete paper: https://tomesphere.com/paper/PMC12128211/full.md

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Source: https://tomesphere.com/paper/PMC12128211