# Application of Radiofrequency Ablation for the Treatment of Cutaneous Hemangioma and Vascular Malformation in a Cockscomb Model

## TL;DR

This study shows that radiofrequency ablation (RFA) is more effective than bleomycin in treating a chicken model of skin vascular conditions, with better results and fewer side effects.

## Contribution

The study introduces a novel histopathological evaluation of RFA in a cockscomb model for cutaneous hemangioma and vascular malformation.

## Key findings

- RFA caused faster and clearer scab formation compared to bleomycin treatment.
- RFA led to a more significant reduction in capillaries and greater collagen proliferation than bleomycin.
- Despite initial differences, capillary numbers in both groups were not significantly different at 28 days.

## Abstract

Background: Radiofrequency ablation (RFA) is an emerging technology for the effective treatment of cutaneous hemangioma and vascular malformation. However, there are few histopathological studies on the treatment of this disease with RFA.

Objective: This study aimed to investigate the effect of RFA and associated histopathological changes in a cockscomb model of cutaneous hemangioma and vascular malformation.

Methods: Thirty-two Leghorn chickens were randomly divided into two groups: RFA group (treated with RFA; 220 V, pulse rate: 15 ms) and control group (treated with 1 mg/mL bleomycin). At 3, 7, 14, and 28 days after treatment, histopathological changes in the cockscomb tissues were observed visually and microscopically using hematoxylin and eosin staining and Masson's trichrome staining. The rates of capillary reduction and collagen proliferation were examined.

Results: The cockscomb in the RFA group developed scabs earlier than that in the bleomycin group, and the scabs were darker and more clearly defined. The RFA group showed a more severe inflammatory reaction than the bleomycin group. At 28 days, most scabs had fallen off in both groups, and the boundary was clearer in the RFA group. At 3, 7, and 14 days, the number of capillaries decreased in both groups, with a more obvious decrease in the RFA group. From Days 3 to 28, the number of capillaries in the RFA group showed a trend of gradual increase, whereas that in the bleomycin group showed a trend of gradual decrease, but there was no significant difference between the two groups at 28 days (p > 0.05). The collagenous fibers of cockscomb showed a trend of gradual increase in both groups. The collagenous fiber hyperplasia was higher in the RFA group than in the bleomycin group at 14 and 28 days (p < 0.01).

Conclusion: RFA significantly reduced the capillary number and promoted tissue fibrosis. Compared with bleomycin, RFA showed a better effect and with no obvious side effects in treating a cockscomb model of cutaneous hemangioma and vascular malformation.