# Study on the Chemical Composition, Biological Activity, and Mechanism of Intervention in Erectile Dysfunction of Healthy Snow Lotus Wine

## TL;DR

This study explores how Healthy Snow Lotus Wine may help treat erectile dysfunction by analyzing its chemical makeup and biological effects.

## Contribution

The study identifies key bioactive compounds and mechanisms in Healthy Snow Lotus Wine that may treat erectile dysfunction.

## Key findings

- Healthy Snow Lotus Wine contains compounds linked to ED-related proteins like STAT3 and AKT1.
- The wine shows high antioxidant capacity and higher levels of phenolics and flavonoids than other samples.
- Network pharmacology and molecular docking reveal multiple pathways and targets involved in ED treatment.

## Abstract

The objective of this study was to investigate the chemical composition, biological activity, and intervention mechanism of Healthy Snow Lotus Wine in the treatment of erectile dysfunction (ED). A preliminary relative characterization and relative quantification of Healthy Snow Lotus Wine was conducted using ultra performance liquid chromatography‐quadrupole orbitrap mass spectrometry (UPLC‐Q‐Orbitrap‐MS). This analysis led to the screening of chemical components that met the criteria of drug similarity and bioavailability. Network pharmacology methods were utilized to identify the active ingredients and their molecular targets of Healthy Snow Lotus Wine that are relevant to the treatment of ED. Enrichment analysis revealed a significant association between Healthy Snow Lotus Wine and pathways related to chemical stress, particularly those involved in cellular basement membrane and phosphatidylinositol 3‐kinase complex signaling. Molecular docking experiments demonstrated that the key compounds bind well to key ED‐related proteins, including STAT3, EGFR, SRC, AKT1, and BCL2. Antioxidant experiments demonstrated that the wine exhibited high antioxidant capacity, with its total phenolic content (TPC), total flavonoid content (TFC), and total proanthocyanidin content (TAC) being superior to those of other samples. These findings provide substantial evidence to support the therapeutic potential of Healthy Snow Lotus Wine in treating ED through multiple mechanisms, underscoring its notable biological activity.

This study investigates the chemical composition, bioactivity, and mechanisms of Healthy Snow Lotus Wine in treating erectile dysfunction (ED). Using UPLC‐Q‐Orbitrap‐MS and network pharmacology, key metabolites and active components were identified, revealing significant antioxidant capacity, lipase inhibition, and multi‐targeted effects on ED‐related proteins. These findings underscore the therapeutic potential of Healthy Snow Lotus Wine for ED treatment through various bioactive pathways.