# Impact of Vitamin E‐Coated Membrane Hemodiafilter on Serum Albumin Redox State in the Acute Kidney Injury Pig Hemodialysis Model

## TL;DR

This study shows that a vitamin E-coated hemodiafilter helps preserve serum albumin and its reduced form in pigs with acute kidney injury during hemodiafiltration.

## Contribution

The novel finding is that vitamin E-coated hemodiafilters minimize the reduction of serum albumin and its reduced form in an acute kidney injury model.

## Key findings

- Serum albumin levels decreased less in the vitamin E-coated group compared to the non-coated group.
- Reduced albumin (RedALB) levels were significantly higher in the vitamin E-coated group.
- A significant positive correlation was found between serum albumin and RedALB levels.

## Abstract

Several studies have evaluated the biocompatibility of dialysis membranes. The use of vitamin E‐coated membranes has been reported multilaterally in in vitro and clinical studies. Nevertheless, the effect of vitamin E‐coated membranes on the redox state of serum albumin, which forms the largest fraction of reactive sulfhydryl groups, has not been reported.

Hemodiafiltration (HDF) with and without a vitamin E‐coated hemodiafilter (V‐RATM group and ABHTM groups, respectively) was performed in an acute kidney injury pig model to determine whether changes in the serum albumin, the oxidized albumin (OxiALB), and the reduced albumin (RedALB) levels differ between the two groups.

Analyses were conducted 22–24 times in the V‐RATM group and 16–18 times in the ABHTM group, excluding missing data. The serum albumin levels decreased in both groups after nephrectomy; however, the decrease observed in the V‐RATM group was significantly lesser than that in the ABHTM group. RedALB levels were significantly higher in the V‐RATM group; in contrast, OxiALB levels did not differ between the two groups. A significant positive correlation was observed between the serum albumin and RedALB levels.

The present study demonstrated that HDF performed using a vitamin E‐coated hemodiafilter effectively minimized the reduction in serum albumin and RedALB levels compared to the vitamin E‐non‐coated hemodiafilter in an acute kidney injury pig model.

The effect of vitamin E‐coated high‐flux hemodiafilter on the redox state of serum albumin, which forms the largest fraction of reactive sulfhydryl, has not been reported. Hemodiafiltration (HDF) with (V‐RATM) and without a vitamin E‐coated hemodiafilter (ABHTM) was performed in an acute kidney injury pig model to determine whether changes in the serum albumin, the oxidized albumin (OxiALB), and the reduced albumin (RedALB) levels differ between the two groups. Vitamin E‐coated hemodiafilter rescued the reduction of serum albumin and RedALB in a pig model with acute kidney injury (AKI), but the vitamin E‐non‐coated hemodiafilter did not minimize and significantly decreased those when compared with baseline. HDF with the vitamin E‐coated hemodiafilter effectively minimized albumin and RedALB in a pig model with AKI.