# Role of Novel Target Molecules (β-Catenin and Retinoblastoma Protein) in the Spectrum of Inflammatory Bowel Disease

## TL;DR

This study explores β-catenin and retinoblastoma protein as potential biomarkers to differentiate between ulcerative colitis and Crohn’s disease in inflammatory bowel disease.

## Contribution

The study introduces β-catenin and retinoblastoma protein as novel immunohistochemical markers for diagnosing subtypes of IBD.

## Key findings

- β-catenin showed 100% sensitivity and 75% specificity in ulcerative colitis cases.
- Retinoblastoma protein demonstrated 75% sensitivity and 95.92% specificity in Crohn’s disease cases.

## Abstract

Background

Inflammatory bowel disease (IBD) has been a worldwide health care challenge with a continually increasing incidence in India. Spectrum of IBD mainly includes ulcerative colitis (UC) and Crohn’s disease (CD). Pharmacological and surgical treatments differ widely in CD and UC; hence, establishment of a correct diagnosis is of paramount importance as it critically influences the disease outcome.

Aim

The aim of this study was to assess the utility and diagnostic accuracy of immunohistochemical profile of β-catenin and retinoblastoma protein in IBD as potential biomarkers.

Study design

This is a cross-sectional observational study.

Material and methods

Data of 61 cases of IBD with UC and CD were retrieved from the Department of Pathology. Immunohistochemical markers β-catenin and retinoblastoma protein were applied on the paraffin blocks of these cases, and their results were interpreted.

Results

Of the 61 cases, 49 cases of UC showed positivity for β-catenin with 100% sensitivity and 75% specificity and nine cases of CD showed positivity for retinoblastoma protein with 75% sensitivity and 95.92% specificity. Three cases were IBD unclassified. Overall, accuracy of β-catenin in UC was found to be 95.08% and that of retinoblastoma protein in CD was 91.80%.

Conclusion

Our study provides insights into the expressions of these specific immunohistochemistry markers and their significance in the differentiation of IBD into UC and CD.