# Scattering polarimetry enables correlative nerve fiber imaging and multimodal analysis

## TL;DR

A new microscope combines two imaging techniques to map nerve fibers in the brain with high resolution and accuracy.

## Contribution

The Scattering Polarimeter integrates 3D-PLI and ComSLI into a single system for correlative and multimodal nerve fiber imaging.

## Key findings

- The Scattering Polarimeter produces results comparable to existing 3D-PLI and ComSLI systems.
- It enables pixelwise mapping of fiber orientations and crossings in human and vervet monkey brain sections.
- The system allows for faster measurements and cross-validation of fiber directions.

## Abstract

Mapping the intricate network of nerve fibers is crucial for understanding brain function. Three-Dimensional Polarized Light Imaging (3D-PLI) and Computational Scattered Light Imaging (ComSLI) map dense nerve fibers in brain sections with micrometer resolution using visible light. 3D-PLI reconstructs 3D-fiber orientations, while ComSLI disentangles multiple directions per pixel. So far, these imaging techniques have been realized in separate setups. A combination within a single device would facilitate faster measurements, pixelwise mapping, cross-validation of fiber orientations, and leverage the advantages of each technique while mitigating their limitations. Here, we introduce the Scattering Polarimeter, a microscope that facilitates correlative large-area scans by integrating 3D-PLI and ComSLI measurements into a single system. Based on a Mueller polarimeter, it incorporates variable retarders and a large-area light source for direct and oblique illumination, enabling combined 3D-PLI and ComSLI measurements. Applied to human and vervet monkey brain sections, the Scattering Polarimeter generates results comparable to state-of-the-art 3D-PLI and ComSLI setups and creates a multimodal fiber direction map, integrating the robust fiber orientations obtained from 3D-PLI with fiber crossings from ComSLI. Furthermore, we discuss applications of the Scattering Polarimeter for unprecedented correlative and multimodal brain imaging.