# Performance evaluation of a novel MBT LipidART module (Bruker Daltonics) for detection of colistin resistance in Escherichia coli and Klebsiella pneumoniae

## TL;DR

This study tests a new tool for quickly detecting colistin resistance in two types of bacteria, finding it works well for one but less so for the other.

## Contribution

The study introduces and evaluates a novel MBT LipidART module for rapid colistin resistance detection in clinical bacteria.

## Key findings

- The module achieved 98.3% categorical agreement for Escherichia coli.
- It showed 85.0% agreement for Klebsiella pneumoniae but faced technical challenges.
- Performance was better for Escherichia coli compared to Klebsiella pneumoniae.

## Abstract

We evaluated the MBT LipidART module on a MALDI Biotyper® Sirius System (Bruker Daltonics) for the rapid detection of colistin resistance in Escherichia coli (EC) and Klebsiella pneumoniae (KPN) by analysing lipid A profiles in negative ion mode. Categorical agreement was achieved for 98.3% EC (N = 58) and 85.0% KPN (N = 40). Challenges included calibration difficulties, limited availability of compatible equipment and issues with mucoid and adherent KPN isolates that yielded invalid results. While MBT LipidART module shows promise as a rapid tool for detection of colistin resistance, its performance was notably better for EC compared to KPN.