# Management of an Equine Herpesvirus-1 Outbreak During a Multi-Week Equestrian Event

## TL;DR

This paper describes how an outbreak of EHV-1 in horses during a large equestrian event was managed through isolation, testing, and biosecurity measures.

## Contribution

The study provides a practical case of EHV-1 outbreak management in a high-risk, ongoing equestrian setting.

## Key findings

- 58% of 38 horses tested positive for EHV-1 during a 28-day monitoring period.
- Only one additional horse developed fever after initial testing.
- Safe return of 17 horses to the event was achieved after two negative PCR tests, 7 days apart.

## Abstract

The present study reports on the management of an EHV-1 outbreak at a large, multi-week equestrian event with ongoing showing. Within a 48 h period, 8 horses out of a cohort of 38 horses from the same trainer displayed elevated rectal temperatures ranging from 38.4 to 39.0 °C. Initial testing using a point-of-care PCR assay detected EHV-1 in 2/8 horses, with the results being confirmed at a later time by qPCR. As a precautionary measure and because of the inability to isolate the entire at-risk population, the 38 horses were relocated to an equine facility outside the equestrian event for daily monitoring and weekly EHV-1 qPCR testing of nasal secretions. Overall, 22/38 (58%) horses tested EHV-1 qPCR-positive in nasal secretions over the monitoring period of 28 days, with only one additional horse developing fever. Once all 38 horses tested EHV-1 qPCR-negative twice, 7 days apart, 17 horses returned to the equestrian event to compete for the remaining 2 weeks of the circuit. The present study highlights the importance of isolating and testing horses with fever but also subfebrile horses, as EHV-1 can cause silent infection. The relocation of the exposed horses to an outside facility allowed close monitoring of these horses while reducing the risk of direct and indirect exposure to other show horses. The regular testing for EHV-1 through nasal secretions during the outbreak, coupled with proper biosecurity protocols, allowed the safe return of the show horses to the event. The key elements in reducing the spread of EHV-1 were the routine assessment of rectal temperature, early isolation of horses with elevated rectal temperature and on-site EHV-1 PCR testing.