# Genetic and Antiviral Potential Characterization of Four Insect-Specific Viruses Identified and Isolated from Mosquitoes in Yunnan Province

## TL;DR

Researchers isolated four insect-specific viruses from mosquitoes in Yunnan and found they may reduce dengue virus levels in cells.

## Contribution

The study identifies four new insect-specific viruses and explores their potential to inhibit dengue virus replication in mosquito cells.

## Key findings

- Four insect-specific viruses (TANV, CxFV, AeFV, LTNV) were isolated from mosquitoes in Yunnan Province.
- Co-infection experiments suggest these viruses may reduce dengue virus (DENV-2) titers in C6/36 cells.
- Insect-specific viruses show potential as biocontrol agents to limit mosquito-borne virus transmission.

## Abstract

Mosquitoes, comprising over 300 species, are pivotal vectors for transmitting arthropod-borne viruses (arboviruses) to vertebrates via bites, posing a significant public health threat with approximately 700,000 annual deaths. In contrast, insect-specific viruses (ISVs) exclusively infect insects and have no direct impact on human health. Yunnan Province in China, located in tropical and subtropical regions, provides an ideal environment for mosquito habitation and has the highest diversity of known mosquito-borne viruses. In this study, mosquito samples were collected from eight cities and states in Yunnan Province, totaling 15,099 specimens. Based on the collection sites and mosquito species, the samples were divided into 110 groups for virus isolation. Four insect-specific viruses (Tanay virus [TANV], Culex orthoflavivirus [CxFV], Aedes orthoflavivirus [AeFV], La Tina virus [LTNV]) were successfully isolated, and co-infection studies with dengue virus (DENV-2) were conducted in C6/36 cells. Preliminary results suggested that these four insect-specific viruses may reduce the viral titer of DENV-2 in C6/36 cells. Understanding the intricate interactions between insect-specific viruses and mosquito-borne viruses is crucial for elucidating the multifaceted role of mosquitoes in arboviral transmission dynamics. Insect-specific viruses exhibit considerable potential as innovative biocontrol agents, with promising capacity to attenuate mosquito-borne viral transmission through the targeted modulation of mosquito innate immunity and physiological adaptations.