Correction: Singano et al. One Health Lens on Rabies: Human–Bat Interactions and Genomic Insights of Rabies Virus in Rural Lilongwe, Malawi. Trop. Med. Infect. Dis. 2025, 10, 95

Abstract

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There was an error in the original publication [1]. A previous proofreading version of the manuscript, which had not incorporated minor text revisions and additional clarifications, was mistakenly submitted.

A correction has been made to multiple sections, Introduction, Materials and Methods, and Results, with minor text updates across several paragraphs to improve clarity and accuracy.

Abstract: RABV genomes from human.

Section 1 (Paragraph 2): RABV in Africa is categorised into lineages 1a, 1b, 2, 3, and 4.

Section 1 (Paragraph 3): children are the most affected.

Section 1 (Paragraph 4): Thus, here, we present factors associated with human–bat interactions and the first whole-genome analysis of rabies virus (RABV) circulating in human and domestic animal (dogs and cattle) hosts in Malawi.

Section 2.4: The PCR conditions included a reverse transcription step at 50 °C for 30 min, followed by an initial denaturation at 94 °C for 2 min. This was followed by 45 cycles at 94 °C for 30 s, annealing at 52 °C for 30 s, and extension at 72 °C for 30 s, with a final extension at 72 °C for 5 min. The resulting amplicons were visualized using a 1.5% agarose gel stained with ethidium bromide with a 100 bp DNA ladder as a size marker.

Section 4.7: Africa 1b subclade.

Section 4.7: The study sequences clustered closely with Zimbabwean, Mozambiquan, Namibian, South African, and Tanzanian Dog sequences, with strong support from significant bootstrap values (>97%). MW36_Lilongwe_Cow/2021 appeared higher in the tree, while MW18_Lilongwe_Dog/2019 and MW03_Blantyre_Human/2019 clustered together, indicating shared ancestry (Figure 2).

We have also added district names to the Malawian sequences throughout the manuscript i.e., from MW03_Human/2019 to MW03_Blantyre_Human/2019.

Section 5 (Paragraph 7 and Paragraph 8): changed MW strains to Malawian strains.

The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.