# On-Site Dual Detection of Airborne Acinetobacter baumannii and Its Carbapenem-Resistant Gene blaOXA-23 Using a One-Pot Visual LAMP-CRISPR/Cas12a-Based Platform

## TL;DR

This paper introduces a fast, on-site method to detect airborne Acinetobacter baumannii and its drug-resistant gene blaOXA-23 using a visual LAMP-CRISPR/Cas12a platform.

## Contribution

A novel one-pot visual LAMP-CRISPR/Cas12a platform for rapid on-site detection of airborne A. baumannii and its resistance gene blaOXA-23.

## Key findings

- The CLC platform can detect airborne A. baumannii and blaOXA-23 within 70 minutes.
- The platform has a detection limit of 6 × 10² CFU of CRAB per test in simulated air samples.
- Results from real hospital air samples matched sequencing data, confirming accuracy and reliability.

## Abstract

Acinetobacter baumannii (A. baumannii), a very common pathogen, poses a significant public health threat due to its antibiotic resistance and long survival in healthcare environments. Both A. baumannii and carbapenem-resistant A. baumannii (CRAB) can spread through the air, increasing infection risks. Therefore, monitoring their presence in the air is of great significance, especially in hospitals. Herein, we developed a Chelex-100-LAMP-CRISPR/Cas12a (CLC) platform including DNA release and nucleic acid test. Combined with a wet cyclone sampler, the platform can detect airborne A. baumannii and its most common carbapenem-resistant gene, blaOXA-23, within 70 min. This CLC platform has also been proven to have a detection limit of 6 × 102 CFU of CRAB per test through simulated air samples. Moreover, this platform was also used to test five actual air samples from a tertiary hospital, and the results achieved perfect concordance with sequencing data, validating the platform’s accuracy and reliability. Therefore, the CLC platform showed great potential for the rapid, on-site detection of airborne A. baumannii and its carbapenem-resistant gene blaOXA-23, offering a valuable tool for infection control in healthcare environments.