Integrative Transcriptome and Metabolome Analysis Reveals Candidate Genes Related to Terpenoid Synthesis in Amylostereum areolatum (Russulales: Amylostereaceae)
Lixia Wang, Ningning Fu, Ming Wang, Zhongyi Zhan, Youqing Luo, Jianrong Wu, Lili Ren
TL;DR
This study identifies genes involved in terpenoid production in the fungus Amylostereum areolatum, revealing key regulators and expanding known terpenoid compounds.
Contribution
The study provides a comprehensive transcriptional framework and identifies key genes regulating terpenoid biosynthesis in Amylostereum areolatum.
Findings
103 terpenoids were identified in Amylostereum areolatum, significantly expanding its known terpenoid repertoire.
26 terpenoid biosynthesis-associated genes were identified, with 11 differentially expressed genes (HMGS1, HMGR2, and AaTPS1-3) highlighted as key regulators.
Triterpenoids and sesquiterpenoids showed the highest diversity and abundance across developmental stages.
Abstract
Amylostereum areolatum (Chaillet ex Fr.) Boidin (Russulales: Amylostereaceae) is a symbiotic fungus of Sirex noctilio Fabricius that has ecological significance. Terpenoids are key mediators in fungal–insect interactions, yet the biosynthetic mechanisms of terpenoids in this species remain unclear. Under nutritional conditions that mimic natural growth, A. areolatum was sampled during the lag phase (day 7), exponential phase (day 14), and stationary phase (day 21). Metabolome (solid-phase microextraction (SPME) combined with gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS)) and transcriptome (Illumina NovaSeq) profiles were integrated to investigate terpenoid–gene correlations. This analysis identified 103 terpenoids in A. areolatum, substantially expanding the known repertoire of terpenoid compounds in this species. Total terpenoid…
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Taxonomy
TopicsPlant biochemistry and biosynthesis · Fungal Biology and Applications · Mycorrhizal Fungi and Plant Interactions