# Improving the Value Utilization of Tuna Peptide Powder for the Cosmetics Field Through Ozone Oxidation

## TL;DR

This study shows how ozone treatment can remove the fishy smell from tuna peptides, making them suitable for use in cosmetics.

## Contribution

A dual-phase ozone treatment is introduced to effectively deodorize tuna peptide powder without affecting its functional properties.

## Key findings

- Wet ozonation at 50°C with 99.1 mg/L ozone for 40 minutes significantly reduces fishy odor.
- n-Hexaldehyde, a key odor compound, decreased by 66.5% after ozone treatment.
- Protein activity in the powder remains unaffected, preserving its functional properties.

## Abstract

The existing in vitro and clinical trial evidence supports the health and wellness benefits of collagen peptides sourced from various origins. Despite this, research on collagen peptides from tuna remains limited. Notably, tuna-derived peptides possess an inherent fishy odor, rendering them unsuitable for direct application in humans. This study explores the enhancement of tuna peptides’ applicability in cosmetics through odor mitigation. We developed a dual-phase ozone treatment, employing both dry and wet ozone, to deodorize tuna peptide powder, enabling its use in cosmetic formulations. The deodorized tuna peptide powder can be used in cosmetics. We optimized the ozone nitrification and deodorization conditions for tuna peptide powder by adjusting the treatment time, ozone concentration, and temperature. Sensory evaluation and GC-MS analysis confirmed the effectiveness of fishy odor removal, offering a comprehensive understanding of the deodorization process. The findings reveal that wet ozonation at 50 °C with an ozone concentration of 99.1 mg/L for 40 min significantly reduces the fishy odor of tuna peptide powder. Notably, n-Hexaldehyde, the primary odor-contributing volatile compound, decreased by 66.5%, confirming the efficacy of ozone treatment in odor mitigation. Moreover, the protein activity within the powder remained unaffected, ensuring the preservation of its functional properties. This study demonstrates the efficacy of ozone oxidation in adapting tuna peptide powder for cosmetic use.