# Phylogenomic and Evolutionary Insights into Lipoprotein Lipase (LPL) Genes in Tambaqui: Gene Duplication, Tissue-Specific Expression and Physiological Implications

## TL;DR

This study explores the evolution and function of lipoprotein lipase genes in tambaqui fish, revealing gene duplication and tissue-specific roles linked to their unique lifestyle and diet.

## Contribution

The study identifies three LPL gene copies in tambaqui and reveals their tissue-specific expression and evolutionary adaptations.

## Key findings

- Three LPL gene copies (lpl1a, lpl1b, lpl2a) were identified in the tambaqui genome.
- lpl1a and lpl2a are highly expressed in the liver, while lpl1b is expressed in the gonads.
- Tambaqui exhibits unique LPL expression patterns linked to its migratory lifestyle and diet.

## Abstract

Background/Objectives: Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism, crucial for the hydrolysis of triglycerides in lipoproteins and maintaining lipid homeostasis in vertebrates. This study aims to characterize the lipoprotein lipase genes in the tambaqui (Colossoma macropomum) genome, investigating their evolutionary history from a phylogenomic perspective. Methods: Phylogenetic and syntenic analyses were used to identify the lpl gene copies in the tambaqui genome and expression patterns were examined across different tissues. A comparative analysis with lpl genes from other vertebrates was also conducted to assess evolutionary relationships and functional diversification. Results: We identified three lpl gene copies in the tambaqui genome: lpl1a, lpl1b, and the lesser-known member of the lipoprotein lipase subfamily, lpl2a. These proteins possess conserved sites essential for lipoprotein lipase function, with variations that may affect their physicochemical properties and lipolytic activity. Key amino acid variations, such as in the lid region and glycosylation sites, were observed among orthologs. Gene expression analysis showed high lpl1a and lpl2a expression in the liver, and lpl1b expression in the gonads, suggesting tissue-specific roles. Comparative analysis revealed distinct expression patterns among teleost fish, with tambaqui exhibiting a unique profile consistent with its migratory lifestyle and varied diet. Conclusions: This study offers new insights into the evolution and functional diversification of lipoprotein lipases in vertebrates, highlighting the complexity of lipid metabolism in fish. These findings contribute to understanding the adaptability of teleost fish to diverse environments and lay the foundation for future research in lipid metabolism regulation, including Neotropical species, with potential applications in aquaculture and conservation.