# The Analysis of Autosomal STRs Draws the Current Genetic Map and Evolutionary History of Northernmost South America

## TL;DR

This study uses genetic markers to map the evolutionary history and genetic diversity of Colombia's population, revealing nine distinct population groups and their ethnic backgrounds.

## Contribution

The study introduces a nine-population classification based on autosomal STRs, integrating genealogical and historical data to explore Colombia's genetic diversity.

## Key findings

- Colombia's genetic diversity is categorized into nine populations, including Afro-descendant, Native American, and Admixed groups.
- Two distinct Afro ancestral components, Chocó and San Andrés, are identified alongside an Afro admixture category.
- Some indigenous groups show preserved genetic configurations, while others exhibit genetic introgression linked to acculturation.

## Abstract

Objectives: To analyze Colombia’s current human population, we employed a population genetics approach enriched by genealogical, demographic, cultural, and historical data to learn about its evolutionary history and to elucidate ethnic belonging and relationship patterns between its various population groups. Materials and Methods: This study relied on ten autosomal microsatellite markers (STRs) from 1364 individuals surveyed throughout the country. Aside from employing descriptive population genetics, substructure, and distance analysis, this investigation evaluated genealogical, demographic, cultural, and historical data gathered from fieldwork surveys. Results: We present a genetic diversity and ethnic belonging map of Colombia that suggests a nine-population classification (under Afro-descendant, Native American, and Admixed ethnicity labels) that reveals traces of evolutionary processes discussed in the light of the recent literature based on modern molecular markers. Colombia’s genetic trace from Africa varies among territories, as shown here by two differentiated Afro ancestral components, Chocó and San Andrés, in addition to the Afro admixture category. Some Native American peoples like the Wayúu, Zenú, Ticuna, Huitoto, and Cocama have a genetic configuration that remains relatively preserved. Nevertheless, other self-determined indigenous peoples who remain in their ancestral territories exhibit genetic introgression that is also reflected by their acculturation levels such as the Pijaos, Kankuamos, and Mokaná. The population classified as European admixture also shows an ancestral component that seems to be more fixed throughout neighboring territories but whose fluctuation depends on its specific demographic histories. Conclusions: This study combines STRs, a targeted sampling strategy, and advanced analytical tools to explore Colombia’s genetic diversity and evolutionary history. Locally, these findings enhance the understanding of genetics in a post-conflict society, crucial for human identification. Globally, they contribute to human population genetics, helping address evolutionary questions using data from diverse human ancestries and geographies.