# Thioflavin T in-gel staining for ex vivo analysis of cardiac amyloid

## TL;DR

This paper introduces a new method using Thioflavin T to stain and analyze amyloid in biological samples, offering a fast and cost-effective approach.

## Contribution

The novel use of Thioflavin T in-gel staining for amyloid analysis is introduced for the first time.

## Key findings

- Thioflavin T in-gel staining is fast, inexpensive, and accessible for most labs.
- The method is compatible with other fluorescent stains and downstream analyses like mass spectrometry.

## Abstract

There are limited options to quantify and characterize amyloid species from biological samples in a simple manner. Thioflavin T (ThT) has been used for decades to stain amyloid fibrils, but to our knowledge, we were the first to use it in-gel. Thioflavin T in-gel staining is convenient as it is fast, inexpensive, accessible to most laboratories, and compatible with other fluorescent stains and downstream analyses such as mass spectrometry (MS).