Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient
Darshan Mehta, Akshay Paradkar, Prakash Nayak, Bharat Rekhi, Bhabani Mohanty, Pradip Chaudhari, Sanjeev K Waghmare
TL;DR
A new cell line from an Indian patient's advanced skin cancer is characterized for its tumor and metastasis traits.
Contribution
A novel hyperdiploid cutaneous squamous cell carcinoma cell line ACSCC1 is established and molecularly characterized.
Findings
ACSCC1 shows increased tumorigenic and metastatic potential with epithelial-mesenchymal transition features.
Whole genome sequencing reveals mutations in cancer-related genes.
TEM analysis shows aberrant mitochondrial morphology and reduced cellular junctions.
Abstract
Cutaneous squamous cell carcinoma (CSCC) is the second most prevalent skin cancer with low metastatic potential; it poses significant morbidity challenges. CSCC possesses significant heterogeneity and the treatment presents a formidable challenge. To gain a clear insight into the diverse nature of these tumors, the development of an in vitro cell line model is essential. However, there are few cell lines that were established, and only one skin SCC cell line is available on the ATCC. In the present study, we established and characterized a novel ACSCC1 cell line from the advanced-stage treatment naïve cutaneous SCC originating from the forearm of the Indian patient. The keratin expression profile showed the epithelial origin of the cell line, ploidy and karyotyping revealed the hyperdiploid population; ACSCC1 showed an increased tumorigenic and metastatic potential. Further, our cell…
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Taxonomy
TopicsCancer Cells and Metastasis · Cancer-related Molecular Pathways · Hippo pathway signaling and YAP/TAZ