# New biomarkers of Kawasaki disease identified by gingival crevicular fluid proteomics

## TL;DR

Researchers identified 12 proteins in gum fluid that could help diagnose Kawasaki disease early, offering new insights into its connection with gum inflammation.

## Contribution

This study is the first to systematically explore GCF proteomics for identifying biomarkers specific to Kawasaki disease.

## Key findings

- 197 differentially expressed proteins were identified in GCF from Kawasaki disease patients compared to controls.
- MRM-MS validated 12 key proteins as potential biomarkers for early diagnosis of Kawasaki disease.
- Functional analysis linked these proteins to immune pathways and highlighted key regulatory proteins like EIF2AK2 and B2M.

## Abstract

Kawasaki disease (KD) is an acute systemic vasculitis that primarily affects coronary arteries, and delayed diagnosis increases the risk of cardiovascular complications. Biomarkers are essential for improving diagnostic accuracy, especially in atypical cases. Gingival crevicular fluid (GCF), derived from periodontal tissues, contains serum components and inflammatory mediators, and has emerged as a valuable biofluid for systemic disease diagnosis. Previous studies suggest GCF protein profiles reflect immune status and metabolic disorders, such as type 2 diabetes. Given the immune-related nature of KD, GCF protein composition may also be altered, yet no studies have systematically explored GCF biomarkers in KD. This study uses DIA and MRM-MS proteomics to identify potential GCF biomarkers for KD diagnosis.

Twenty-seven patients with KD were enrolled in this study, and 18 healthy volunteers were recruited as the control group. GCF samples were collected from the KD patients, who formed the experimental group, before they received intravenous immunoglobulin treatment. Data-independent acquisition (DIA) quantitative proteomics mass spectrometry was performed on the GCF samples to analyze the protein expression profiles in both groups. DEPs were identified and subjected to functional enrichment analysis using KEGG and GO. Protein–protein interaction (PPI) analysis was conducted for all detected DEPs. Finally, multiple reaction monitoring mass spectrometry (MRM-MS) was used to validate the selected DEPs.

A total of 197 DEPs were identified in GCF between the KD group and the normal control group, with 174 upregulated and 23 downregulated proteins. Functional enrichment analysis revealed that cellular and metabolic processes were the most significantly altered biological processes, while binding and catalytic activity were the most affected molecular functions. Pathway analysis further highlighted the NOD-like receptor signaling pathway, protein processing in the endoplasmic reticulum, and the influenza pathway as the most significantly enriched pathways. In the PPI network, EIF2AK2, B2M, and GBP1 were identified as key hub proteins, suggesting their potential regulatory roles in KD pathophysiology. Finally, MRM-MS confirmed the expression patterns of 12 DEPs (IFIT3, UB2L6, HP, A1AT, HSP90AA1, HNRPC, HSP90AB1, SAA1, MX1, B2M, FKBP4, and TRAP1), thereby demonstrating high consistency with the DIA results and further validating the DEPs’ potential as biomarkers for KD.

Our findings suggest that 12 proteins in GCF could serve as potential biomarkers for the early diagnosis of KD. Additionally, the molecular analysis revealed a close association between KD and gingival inflammation, offering new insights into KD’s pathophysiology and potential directions for improved diagnosis and treatment.