# Spatially resolved transcriptomic profiling for glomerular and tubulointerstitial gene expression in C3 glomerulopathy

## TL;DR

This study uses spatial transcriptomics to identify gene expression patterns in C3 glomerulopathy, revealing changes in genes related to the extracellular matrix and immune response.

## Contribution

The study presents the first spatially resolved transcriptomic profiling of C3 glomerulopathy, revealing disease-specific gene expression patterns.

## Key findings

- C3G showed 229 and 157 differentially expressed genes in glomeruli compared to donor and disease controls, including POSTN, COL1A2, and IFI44L.
- Top enriched gene ontology terms in C3G glomeruli included protease binding, structural molecule activity, and extracellular matrix components.
- In vitro validation confirmed that C3G-related genes are upregulated in glomerular endothelial cells when co-cultured with complement-stimulated macrophages.

## Abstract

Complement 3 (C3) glomerulopathy (C3G) is a rare but clinically significant glomerulopathy. However, little is known about its transcriptomic profile. We investigated the substructure-specific gene expression profile of C3G using the recently introduced spatial transcriptomics technology.

We performed spatial transcriptomic profiling using GeoMx Digital Spatial Profiler with formalin-fixed paraffin-embedded kidney biopsy specimens of three C3G cases and seven controls from donor kidney biopsy. Additionally, 41 samples of other glomerulonephritis, including focal segmental glomerulosclerosis, membranous nephropathy and minimal change disease, were included as disease controls. We identified differentially expressed genes (DEGs) specific to C3G, followed by in vitro validation analysis of consistently upregulated DEGs in human glomerular endothelial cells through a co-culture with complement-stimulated macrophages.

We found 229 and 157 highly expressed DEGs in the glomeruli of C3G compared with those of donor and disease controls, respectively, including POSTN, COL1A2 and IFI44L. Protease binding, structural molecule activity and extracellular matrix (ECM) structural constituent were among the top enriched Gene Ontology terms in the glomeruli of C3G. Specifically, genes related to the ECM and interferon activity were the most upregulated, with network analysis suggesting possible interactions between complement C3 and the ECM through CD11c. The in vitro experimental validation using iC3b-stimulated CD11c+ macrophages supported these findings, inducing elevated expression of fibrosis markers and ECM components in glomerular endothelial cells.

Significant disease-specific transcriptomic alterations in C3G, including upregulation of genes related to the ECM, provide potential insights into the pathophysiology.

Graphical Abstract