# Diurnal Variation of 8-hydroxy-2’-deoxyguanosine in Continuous Time Series of Two Breast Cancer Survivors

## TL;DR

The study shows that 8-OHdG levels in urine vary significantly between day and night in breast cancer survivors, highlighting the need for timed measurements in clinical assessments.

## Contribution

The study demonstrates diurnal variation in 8-OHdG levels in breast cancer survivors using continuous time series data.

## Key findings

- 8-OHdG levels were significantly higher during the day than at night in both subjects.
- Night samples showed higher variability in 8-OHdG levels compared to day samples.
- Day-night differences were consistent regardless of creatinine or volume correction.

## Abstract

8-hydroxy-2’deoxyguanosine (8-OHdG) is an oxidative product removed from DNA following radical oxygen species-induced damage. As a water-soluble molecule, it can be measured non-invasively in urine and is commonly used as a marker for ‘whole-body’ oxidative stress. However, its validity and reliability in assessing oxidative stress across various chronic diseases and in early carcinogenesis screening in clinical diagnosis and research remain subjects of debate. To determine optimal measurement timing and duration, it is essential to establish the circadian profile of 8-OHdG under everyday life conditions. Here, applying the integrative single-case design, we show the presence of day-night differences for 8-OHdG in continuous time series of two breast cancer survivors while considering different correction approaches. The participants sampled their urine in 12-h-pooled collections over one month. In both subjects, 8-OHdG levels were significantly higher during the day than at night regardless of whether they were corrected by creatinine or urine volume (creatinine corrected: t = –6.43, p < 0.01 [subject 1], t = –2.69, p = 0.01 [subject 2]; volume corrected: t = –7.30, p < 0.01 [subject 1], t = –3.69, p < 0.01 [subject 2]). Notably, urinary 8-OHdG levels show higher variability in night samples compared to day samples. These findings underscore the importance of considering daily fluctuations in 8-OHdG levels in both clinical and research studies, as well as the need to account for the dynamic characteristics of stress markers to minimize the risk of inconsistent or misleading results in clinical diagnostics.