Integrating Transcriptomic and Proteomic Data: IL‐27B as a Key Protein in the Development of Septic Cardiomyopathy—A Retrospective Study

TL;DR

This study combines transcriptomic and proteomic data to identify IL-27B as a key protein in septic cardiomyopathy, offering new insights into its mechanisms and potential treatments.

Contribution

The novel integration of transcriptomic and proteomic data reveals IL-27B's role in septic cardiomyopathy progression.

Findings

IL-27B and carbonic anhydrase are co-downregulated in septic cardiomyopathy patients.

IL-27B is linked to immune response and cytokine signaling pathways.

Integrated analysis highlights inflammation and amino acid metabolism in SCM pathology.

Abstract

Septic cardiomyopathy (SCM) is a potentially fatal complication of sepsis. In this study, transcriptomic and proteomic analyzes of serum samples from sepsis patients were conducted to uncover the underlying pathological mechanisms and identify potential therapeutic targets for SCM.

This retrospective, dual‐center study investigated the progression of sepsis to SCM in patients admitted to intensive care units. A total of 50 patients were enrolled and divided into two groups: sepsis with cardiomyopathy (25 cases) and sepsis without cardiomyopathy (25 cases). Co‐expression network analysis was employed to elucidate the biological significance of differentially expressed proteins. By integrating proteomic and transcriptomic data, molecular networks were constructed to visualize interactions among key molecules, aiming to enhance data interpretation and support the study's findings.

Proteomic analysis identified 216 differentially expressed proteins (Fold change > 1.5, p‐value < 0.05) between the two groups. Transcriptomic analysis revealed two proteins, including Interleukin‐27 subunit beta (IL‐27B) and carbonic anhydrase, co‐downregulated in patients with septic cardiomyopathy. IL‐27B was associated with the immune response, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated its involvement in the cytokine‐cytokine receptor interaction signaling pathway.

Comprehensive integrated transcriptomic and proteomic analyzes identified significant changes in protein expression associated with SCM, primarily associated with inflammation‐related pathways and amino acid metabolism. These findings provide new insights into the pathological mechanisms of SCM and highlight potential therapeutic targets for its treatment.

The Clinical Research Ethics Committee of Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University approved this study, and written informed consent was given by all patients or their legal representatives. (NO.K20201110).

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