Strategies for improved endothelial cell adhesion in microphysiological vascular model systems
Jingyi Zhu, Halie L. Hotchkiss, Kevin L. Shores, George A. Truskey, Stacey A. Maskarinec, Ahmed El-Fiqi, Ahmed El-Fiqi, Ahmed El-Fiqi, Ahmed El-Fiqi
TL;DR
This paper describes a protocol to improve the adhesion of endothelial cells in engineered blood vessels, enhancing their use in studying vascular diseases.
Contribution
The study introduces an optimized protocol for endothelial cell adherence in TEBVs, including a redesigned chamber for monitoring.
Findings
Optimized seeding density, rotation time, and perfusion improve endothelial cell coverage in TEBVs.
Redesigned TEBV chambers with a viewing window allow efficient monitoring of endothelialization.
The endothelialized TEBVs show alignment with flow and response to inflammatory stimuli.
Abstract
Human tissue-engineered blood vessels (TEBVs) have been applied as model systems to study a wide range of vascular diseases including Hutchinson-Gilford Progeria Syndrome and early atherosclerosis. Central to the utility of TEBVs as an in vitro blood vessel model is the maintenance of a functional endothelium under physiologically relevant shear stresses. Establishing and maintaining a confluent endothelial monolayer is challenging. In this protocol, we outline an optimized procedure for the endothelialization of TEBVs. We optimized the following key conditions affecting endothelial cell (EC) adherence in the vessel: EC seeding density, rotation time, and the application of perfusion. This protocol results in TEBVs with sustained EC luminal coverage that demonstrate alignment in the direction of applied flow and responsiveness to inflammatory stimuli. To facilitate rapid screening of EC…
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Taxonomy
TopicsRenal and related cancers · Congenital heart defects research · 3D Printing in Biomedical Research