# Narrow Versus Broad Phenotype Definitions Affect Genetic Analysis of Language More Than Other Broad Autism Phenotype Traits

## TL;DR

This study shows that using narrow definitions of autism and language traits leads to more precise genetic findings than broader definitions.

## Contribution

The study identifies specific genetic regions and candidate genes linked to language and reading impairments in autism using refined phenotypic criteria.

## Key findings

- Narrow autism and language impairment definitions yielded higher linkage probabilities (57% and 33%) than broader definitions.
- Ten and six top candidate genes were identified for language and reading impairments, respectively.
- Some genes, like ZNF774 and DNAH3, are newly implicated in neurodevelopmental phenotypes.

## Abstract

Autism spectrum disorder is a heritable neurodevelopmental condition that displays heterogeneity in both presentation and etiology and it often presents with concomitant communication difficulties. The hypothesis behind the New Jersey Language and Autism Genetic Study is that genetic heterogeneity for component phenotypes of autism spectrum disorder may be reduced relative to the disorder as a whole. We previously published an initial phase of this study with family recruitment that used very restricted inclusion/exclusion criteria for both autism and language deficits in other family members. Here we present an expanded sample that includes a wider range of phenotypic presentations in the autism and language domains. We found that our previous findings on 15q and 16q, connecting autism spectrum disorder and oral/written communication, are only relevant to the narrow autism spectrum disorder and language impairment phenotypes (with posterior probability of linkage of 57% and 33%, respectively) though addition of families did reduce both critical regions. After variant prioritization and additional filtration based on segregation patterns and functional annotations, we determined a set of 10 and 6 top candidate risk genes with strong association to language impairment and reading impairment, respectively. The top candidate genes include both genes previously implicated in neurodevelopmental disorders (e.g., ZNF774 and DNAH3) and genes not previously reported but with strong evidence of being involved in neurodevelopmental phenotypes.