# Respiratory Virus Detection and Sequencing from SARS-CoV-2–Negative Rapid Antigen Tests **Authors:** Emmanuela Jules, Charlie Decker, Brianna Jeanne Bixler, Alaa Ahmed, Zijing (Carol) Zhou, Itika Arora, Henok Tafesse, Hannah Dakanay, Andrei Bombin, Ethan Wang, Jessica Ingersoll, Kathy Bifulco, Jennifer K. Frediani, Richard Parsons, Julie Sullivan, Morgan Greenleaf, Jesse J. Waggoner, Greg S. Martin, Wilbur A. Lam, Anne Piantadosi PMC · DOI: 10.3201/eid3113.241191 · Emerging Infectious Diseases · 2025-05-01 ## TL;DR This paper shows how to detect and sequence various respiratory viruses using tests that initially come back negative for SARS-CoV-2. ## Contribution The novel approach uses metagenomic sequencing on negative rapid antigen tests to enable broad respiratory virus surveillance. ## Key findings - Metagenomic sequencing of negative SARS-CoV-2 tests identified multiple respiratory viruses. - Full genome sequences were successfully generated from these tests. - This method streamlines genomic surveillance for a wide range of respiratory viruses. ## Abstract Genomic epidemiology offers insight into the transmission and evolution of respiratory viruses. We used metagenomic sequencing from negative SARS-CoV-2 rapid antigen tests to identify a wide range of respiratory viruses and generate full genome sequences. This process offers a streamlined mechanism for broad respiratory virus genomic surveillance. ## Linked entities - **Diseases:** SARS-CoV-2 (MONDO:0100096) ## Full-text entities - **Species:** Severe acute respiratory syndrome coronavirus 2 (no rank) [taxon 2697049] ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12078552/full.md ## Figures 3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12078552/full.md ## References 10 references — full list in the complete paper: https://tomesphere.com/paper/PMC12078552/full.md --- Source: https://tomesphere.com/paper/PMC12078552