# A haplotype-resolved genome assembly and gene expression map of Cushion willow

**Authors:** Jindan Wang, Kaiyun Chen, Rengang Zhang, Yuan Huang, Jiahui Chen

PMC · DOI: 10.1038/s41597-025-05132-3 · Scientific Data · 2025-05-13

## TL;DR

This paper presents a high-quality, haplotype-resolved genome and gene expression map for Cushion willow, a plant adapted to alpine environments.

## Contribution

The study provides a near telomere-to-telomere genome assembly and a detailed transcriptomic map for Cushion willow.

## Key findings

- The Cushion willow genome is now haplotype-resolved and nearly complete.
- A high-quality gene expression atlas was created to support future research.
- The updated genome enhances potential for studying alpine adaptation and sex determination.

## Abstract

Salix brachista, commonly known as Cushion willow, is a common component of subnival alpine assemblages and a dioecious or monoecious plant with a creeping stem and numerous lateral branches. Cushion willow takes cuttings more easier and has a specific sex system, making it a suitable system for studying the evolution of plant sex determination, adaptive evolution of alpine plants, and mining stress resistance gene resource that cope with the hostile alpine environment. Therefore, Cushion willow has potential value in genetic improvements for willows used as bioenergy crops, in gardening, and as ornamental plants. However, the genome of Cushion willow still contains some un-assembled repetitive sequences, and there is limited availability of a gene expression atlas, which hinders its potential use for the aforementioned purposes. Here, we updated the genome of Cushion willow to be haplotype-resolved and near telomere-to-telomere, and obtained a high-quality transcriptomic map. Our research provides a potential model species for alpine adaptive research, sex determination evolution studies, and improving willow crops.

## Linked entities

- **Species:** Salix brachista (taxon 2182728)

## Full-text entities

- **Species:** Salix brachista (species) [taxon 2182728], Salix (willows, genus) [taxon 40685]

## Full text

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## Figures

6 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12075806/full.md

## References

7 references — full list in the complete paper: https://tomesphere.com/paper/PMC12075806/full.md

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Source: https://tomesphere.com/paper/PMC12075806