# Staphylococcus epidermidis SAS1: new probiotic candidate for obesity and allergy treatment their mechanistic insights and cytotoxicity evaluation

## TL;DR

This study explores a new probiotic, Staphylococcus epidermidis SAS1, for its potential to treat obesity and allergies by regulating immune responses and reducing fat accumulation.

## Contribution

The study introduces SAS1 as a novel probiotic candidate with dual therapeutic potential for obesity and allergies.

## Key findings

- SAS1 showed antiallergic potential by reducing interleukins and increasing reactive oxygen species.
- It exhibited anti-obesity effects by inhibiting lipase and reducing LDL and triglyceride levels.
- Cytotoxicity tests confirmed its safety at effective concentrations.

## Abstract

Probiotics are live bacteria that provides numerous healthy and beneficial effects to the consumers. The present study aimed to investigate the effect of a probiotic candidate Staphylococcus epidermidis SAS1, in immunoregulation and obesity management.

: This probiotic candidate was isolated from a soil sample collected from a region of fruit waste decomposition. In vitro cytotoxicity was assessed using the THP-1 (human leukemia monocytic cell line) cells using MTT assay.

An IC50 value of 47.52 ± 0.18 μg/mL and cell shrinkage were observed along with the release of cellular content of THP-1 cells. The higher production of reactive oxygen species and lesser release of interleukins (IL-4, 5, and 13) are attributed to the antiallergic potential of this strain. Furthermore, in vitro cytotoxicity evaluation using 3T3-L1 cells identified this strain as a promising candidate for anti-obesity treatment. The observed IC50 value was 514.4 ± 0.061 μg/mL.

This extract was shown to have good lipase-inhibiting enzyme activity and was reported to prevent adipogenesis, depicted by increased HDL levels and decreased LDL and triglyceride levels. These results suggested that Staphylococcus epidermidis SAS1 may have therapeutic use in the treatment of obesity and allergies.