# Fabricating Natural Polymeric Encapsules for Pest Control Uploaded with 1,8-Cineole Extracted from Eucalypt Ecotypes’ Leaves Using Innovative Microwave Tool

## TL;DR

This study explores using eucalypt essential oil in natural encapsules for pest control, finding that higher concentrations of 1,8-cineole increase termite mortality.

## Contribution

The study introduces a novel microwave-assisted method for extracting eucalypt essential oil and evaluates its encapsulation for pest control applications.

## Key findings

- Eucalypt leaves from Hada Al-Sham village had the highest 1,8-cineole content (59.29%).
- Microwave-assisted steam distillation (MASD) is more eco-friendly and effective than traditional methods.
- Encapsules with higher 1,8-cineole concentrations significantly increased termite mortality.

## Abstract

This research explores the potential of green encapsules uploaded with eucalypt essential oil (EEOs) in enhancing their functionality and application in pest control, focusing on suitable ecotype selection from King Abdulaziz University (KAU) campus, Hada Al-Sham (HAS) village, and Briman district as well as optimizing extraction processes. Eucalypt hybrids’ leaves were collected from three different sites, and the EEOs were extracted using microwave-assisted steam distillation (MASD) and electric steam distillation (ESD) techniques. The physical and chemical properties of the EEO were determined. The identification of volatile chemical ingredients in the resulting EEOs was conducted using GC/MS after saponification and methylation procedures, and the ingredients were compared to those obtained from Eucalyptus globulus Labill, the ideal species containing the 1,8-cineol, the principal compound in its essential oil. The 1,8-cineole was found to be the major chemical constituent of the EEOs all over the two extraction methods, regardless of the ecotypes examined, and was interfered with other minor components such as 3-carene, α-pinene, α-myrcene, D-limonene, and α-terpinene. Eucalypt ecotypes grown at Hada Al-Sham village had the highest cineole content (59.29%) among the other sites studied. Compared to the ESD technique, MASD showed much promise because it is simple, facile, more ecofriendly and cost-effective, it kept oils true to their original form, and it allows to warm larger machines and spaces. The polymeric encapsules of either guar gum crosslinked by borax or sodium alginate crosslinked by calcium chloride were fabricated. Moreover, a bioassay screening of the encapsules uploaded with 1,8-cineole was evaluated against termite infection. The encapsules were found to be versatile tools with a wide range of applications; in particular, the alginate encapsules displayed superior characteristics. Furthermore, regardless of the encapsule type and the exposure duration, the mortality (%) of the insects was exceeded significantly for the high cineol concentrations compared to the lower ones for both alginate-based encapsules (ABEs) and guar gum-based encapsules (GGBEs). The higher the cineol concentrations, the higher the mortality percent of the termites. This finding can be attributed to the rapid toxic effect of the cineol compound at higher concentrations.