# Genome Announcement of Four Parechovirus A3 Isolates from the United States of America

**Authors:** Debarpan Dhar, Terry Fei Fan Ng, Christopher J. Harrison, Eric Rhoden, Bernardo A. Mainou, Anjana Sasidharan, Katelyn E. VanDonge, Varun Chandra Boinpelly, Rangaraj Selvarangan

PMC · DOI: 10.3390/ijms26094378 · International Journal of Molecular Sciences · 2025-05-05

## TL;DR

This paper announces the complete genome sequences of four Parechovirus A3 isolates from the USA, highlighting genetic differences and phylogenetic clustering.

## Contribution

The study provides new genome sequences and identifies unique mutations and phylogenetic patterns in PeV-A3 isolates.

## Key findings

- Multiple mutations were found in PeV-A3 isolates compared to the reference sequence.
- 2022 isolates showed unique amino acid changes not present in the 2012 isolate.
- 2022 isolates formed a distinct phylogenetic clade separate from the 2012 isolate.

## Abstract

We report the complete genome sequences of four parechovirus-A3 (PeV-A3) isolates from Children’s Mercy Kansas City (CMKC), United States of America (USA): PeV-A3-MO-12-CMKC/CSF/MO/USA/2012 (isolated in 2012 from cerebrospinal fluid), PeV-A3-8C-CMKC/CSF/MO/USA/2022/ (isolated in 2022 from cerebrospinal fluid), PeV-A3-9C-CMKC/CSF/MO/USA/2022 (isolated in 2022 from cerebrospinal fluid), and PeV-A3-11B-CMKC/Blood/MO/USA/2022 (isolated in 2022 from blood). Sequence analysis revealed multiple mutations throughout the genome of the PeV-A3 isolates in comparison to the prototypic PeV-A3 A308/99 reference sequence (AB084913). Several unique amino acid changes were observed in the PeV-A3 isolates from 2022 that were absent in the PeV-A3 isolate from 2012. Phylogenetic analysis comparison determined that the sequenced PeV-A3 isolates from 2022 cluster together as a separate clade.

## Full-text entities

- **Diseases:** gastroenteritis (MESH:D005759), respiratory infections (MESH:D012141), injury to (MESH:D014947), PeV-A3 (MESH:C537090), infected (MESH:D007239), sepsis (MESH:D018805), like illness (MESH:C537675)
- **Chemicals:** Streptomycin (MESH:D013307), Penicillin (MESH:D010406), EMEM (-)
- **Species:** Peromyscus eva (species) [taxon 144756], Parechovirus (genus) [taxon 138954], Homo sapiens (human, species) [taxon 9606], Parechovirus A (no rank) [taxon 1803956]
- **Mutations:** L776P, 2A-C, E1966D, V832I, I2060V, E1440D, I1714V, I1577V, S1129A, S1958A, G2059N, N1206D, K781N, Q1355E, Q1348K, V1466A, S1507T, A765V, S1418N, A1463V, V915I, K1482R, S1811T, R764G, A767V, I1178V, A1766S, Y1943F, D1976N, R1426K, A1858T, N877S, T1322I, K1344Q, A1498V, V885I, S1620N, A1311S
- **Cell lines:** Vero-P — Chlorocebus sabaeus (Green monkey), Spontaneously immortalized cell line (CVCL_0059)

## Full text

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## Figures

5 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12072549/full.md

## References

24 references — full list in the complete paper: https://tomesphere.com/paper/PMC12072549/full.md

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Source: https://tomesphere.com/paper/PMC12072549