# Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology

## TL;DR

This study explores how asymmetric comb structures made of tantalum and silicon oxide affect the alignment and shape of mammalian cells.

## Contribution

The paper introduces new insights into how asymmetric comb structures influence cell behavior, particularly with varying line and trench widths.

## Key findings

- Trench and line widths are critical design parameters affecting cell behavior on asymmetric comb structures.
- Replacing tantalum lines with silicon oxide reduces the ability to manipulate cell morphology.
- Cell orientation and morphology were analyzed using fluorescence confocal and scanning electron microscopy.

## Abstract

Mammalian tissues and cells often orient naturally in specific patterns to function effectively. This cellular alignment is influenced by the chemical nature and topographic features of the extracellular matrix. In implants, a range of different materials have been used in vivo. Of those, tantalum and its alloys are promising materials, especially in orthopedic implant applications. Previous studies have demonstrated that nano- and micro-scale surface features, such as symmetric comb structures, can significantly affect cell behavior and alignment. However, patterning need not be restricted to symmetric geometries, and there remains a gap in knowledge regarding how cells respond to asymmetric comb structures, where the widths of the trenches and lines in the comb differ. This study aims to address this gap by examining how Vero cells (cells derived from an African green monkey) respond when applied to tantalum and tantalum/silicon oxide asymmetric comb structures having fixed trench widths of 1 μm and line widths ranging from 3 μm to 50 μm. We also look at the cell responses on inverted patterns, where the line widths were fixed at 1 μm while trench widths varied. The orientation and morphology of the adherent cells were analyzed using fluorescence confocal microscopy and scanning electron microscopy. Our results indicate that the widths of the trenches and lines are important design parameters influencing cell behavior on asymmetric comb structures. Furthermore, the ability to manipulate cell morphology using these structures decreased when parts of the tantalum lines were replaced with silicon oxide.