Impact of Allele-Specific Expression on Ripening and Quality Characteristics of ABB Banana Fruit

TL;DR

This study explores how allele-specific expression affects ripening and quality traits in ABB bananas, revealing key genes involved in processes like ethylene biosynthesis and starch degradation.

Contribution

The study identifies ASE patterns in ripening-related pathways and highlights key ASE genes in ABB bananas that influence fruit quality.

Findings

27.2% and 22.2% of DEGs showed ASE in Fen Jiao bananas at 3 and 6 days postharvest.

ASE genes were more frequent in ripening-related pathways like ethylene biosynthesis and starch degradation.

B alleles of key enzyme genes (ACS/ACO/AMY) were more active than A alleles in Fen Jiao bananas.

Abstract

Allele-specific expression (ASE) is a phenomenon in which the expression level of an allele from both parents is inconsistent, which is considered to play a key role in the differences between hybrids. As a typical climacteric fruit, banana undergoes a ripening process that affects the quality of the fruit. BaXi (Musa, AAA group) and Fen Jiao (Musa, ABB group) banana fruits show different traits during postharvest ripening, and their high-quality reference genomic sequences have been published. In this work, we analyzed differentially expressed genes (DEGs) in these two banana cultivars based on the transcriptomes during the postharvest stages. Additionally, the imbalance expression of alleles of DEGs in Fen Jiao banana fruit was analyzed, revealing that 27.2% (3 d) and 22.2% (6 d) of the 15,415 DEGs showed ASE. Then, the ASE profiles related to the post-ripening of banana fruit were built, focusing on ripening-related pathways, such as ethylene biosynthesis (62.5–83.3%), starch degradation (0–75%) and cell wall material degradation (34.6–90.9%). The ASE genes involved in ripening were more frequent than those associated with general gene expression. In addition, the candidate key genes of ASE alleles involved in ethylene synthesis and starch degradation were identified, including the alleles of MaACS7/MbACS7, MaACO2/MbACO6, MaACO3/MbACO7, MaACO8/MbACO13 and MaACO6/MbACO17 involved in ethylene biosynthesis, and those of MaAMY1/MbAMY3, MaBMY1/MbBMY2, MaBMY7/MbBMY8 and MaDPE2/MbDPE2 involved in starch degradation. The expression of the B genes of these key enzyme genes (ACS/ACO/AMY) is more active than that of the A genes in Fen Jiao bananas.

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