# The Influence of Moringa Oleifera Leaf Powder on Body Weight MYF5 and IGF‐1gene Expression in Japanese Quails

## TL;DR

This study shows that adding Moringa leaf powder to quail diets can improve growth and gene activity linked to muscle development.

## Contribution

The study demonstrates that Moringa oleifera leaf powder can enhance poultry growth and gene expression related to muscle development.

## Key findings

- Quails fed with 4% and 6% Moringa leaf powder had significantly higher body weight and feed efficiency.
- MYF5 and IGF-1 gene expression increased in a dose-dependent manner with higher Moringa leaf powder levels.

## Abstract

This study investigates the effects of Moringa oleifera leaf powder (MOLP) supplementation on growth performance and the expression of MYF5 and IGF‐1 genes in Japanese quails. A total of 116 quails were assigned to four dietary treatments: Control, Moringa‐1 (2% MOLP), Moringa‐2 (4% MOLP), and Moringa‐3 (6% MOLP). Body weights were recorded weekly, and gene expression levels were analysed using quantitative real‐time PCR. The results showed significant improvements in body weight and feed efficiency in the Moringa‐2 and Moringa‐3 groups compared to the Control group (p < 0.05). Additionally, the expression levels of MYF5 and IGF‐1 increased dose‐dependent, with the highest expression observed in the Moringa‐3 group. These findings demonstrate the potential of MOL as a natural feed additive to enhance growth performance and modulate molecular pathways related to muscle development in poultry. This study provides valuable insights for sustainable poultry production and highlights the need for further research on optimal inclusion levels and the long‐term effects of MOL supplementation.

This study investigates the effects of Moringa oleifera leaf powder (MOLP) supplementation on growth performance and the expression of MYF5 and IGF‐1 genes in Japanese quails. A total of 116 quails were assigned to four dietary treatments: Control, Moringa‐1 (2% MOLP), Moringa‐2 (4% MOLP), and Moringa‐3 (6% MOLP). Body weights were recorded weekly, and gene expression levels were analysed using quantitative real‐time PCR. The results showed significant improvements in body weight and feed efficiency in the Moringa‐2 and Moringa‐3 groups compared to the Control group (p < 0.05). Additionally, the expression levels of MYF5 and IGF‐1 increased dose‐dependent, with the highest expression observed in the Moringa‐3 group. These findings demonstrate the potential of MOL as a natural feed additive to enhance growth performance and modulate molecular pathways related to muscle development in poultry. This study provides valuable insights for sustainable poultry production and highlights the need for further research on optimal inclusion levels and the long‐term effects of MOL supplementation.