# Evaluation of LncRNAs CBR3-AS1 and PCA3 expression in Gastric cancer and their correlation to clinicopathological variables

## TL;DR

This study examines the expression of two long non-coding RNAs, CBR3-AS1 and PCA3, in gastric cancer and finds they are elevated in tumor tissues, suggesting potential roles as biomarkers.

## Contribution

The study identifies elevated CBR3-AS1 and PCA3 expression in gastric cancer tissues compared to healthy tissues, proposing their potential as diagnostic biomarkers.

## Key findings

- PCA3 and CBR3-AS1 lncRNAs are significantly overexpressed in gastric cancer tumor tissues compared to adjacent healthy tissues.
- The AUC values of 0.68 and 0.79 for PCA3 and CBR3-AS1 suggest moderate diagnostic potential for gastric cancer.
- No significant correlation was found between lncRNA expression and clinicopathological variables.

## Abstract

Background: Gastric cancer (GC) is a multifactorial disease with a high death rate due to the unknown mechanisms involved in the developing, progressing, and late diagnosing GC. Several cancers have been linked to Long non-coding RNAs (lncRNAs), including GC, through differential expression. They play a crucial role in tumorigenesis pathways as modulatory factors, making them intriguing clinical and diagnostic biomarkers for many malignancies. This study’s objective is to compare the lncRNAs CBR3-AS1 and PCA3 expression levels in tumoral tissues to marginal tissues and the clinicopathological features of patients.

Methods and Results: 100 GC patients’ tumoral and marginal tissue samples from Tabriz’s Valiasr Hospital were gathered for this case-control research. To determine the expression level of PCA3 and CBR3-AS1 lncRNAs in GC, total RNA was extracted, and the qRT-PCR technique was employed. Compared to adjacent marginal tissues, the tumor tissue of patients with GC showed a significant increase in the expression levels of PCA3 and CBR3-AS1 (P < 0.0001). The expression ratio of lncRNA CBR3-AS1 and PCA3 did not significantly correlate with clinicopathological variables. The ROC curve’s findings lead to the conclusion that the genes lncRNAs PCA3 and CBR3-AS1, with AUC values of 0.68 and 0.79, respectively, suggest that they could play carcinogenic roles in GC and may act as moderate diagnostic biomarkers for GC.

Conclusions: In GC, CBR3-AS1 and PCA3 may be utilized as therapeutic targets and prognostic biomarkers, respectively.