# CRISPR/Cas9-based modulation of V-PPase expression in rice improves grain quality and yield under high nighttime temperature

## TL;DR

Modifying a specific enzyme in rice using CRISPR/Cas9 improves grain quality and fertility when nighttime temperatures are high.

## Contribution

A novel CRISPR/Cas9-based approach to enhance rice grain quality under high nighttime temperatures.

## Key findings

- Modulating V-PPase expression improves spikelet fertility in rice.
- Reduced grain chalkiness is observed under high nighttime temperatures.
- The method specifically targets endosperm and reproductive tissues.

## Abstract

Transcriptional modulation of the vacuolar H+ translocating pyrophosphatase expressed specifically in the endosperm and reproductive tissue of rice improves its spikelet fertility and reduces grain chalkiness under high nighttime temperature.

The online version contains supplementary material available at 10.1007/s00299-025-03504-y.