# Repeated CD107a Staining Enables Identification of Serial Degranulating NK Cells

**Authors:** Jens Niemann, Maren Claus, Vanna Imširović, Carsten Watzl

PMC · DOI: 10.1002/eji.202451642 · European Journal of Immunology · 2025-05-09

## TL;DR

This paper introduces a method to identify natural killer cells that repeatedly release granules by using multiple CD107a antibody stains.

## Contribution

A novel flow-cytometry-based method to identify serial degranulating NK cells through repeated CD107a staining.

## Key findings

- Serial degranulating NK cells can be identified by multiple CD107a staining events.
- The method enables characterization and isolation of these cells for further study.

## Abstract

Upon repeated target cell contact serial degranulating NK cells are identified by multiple staining events using differentially labeled anti‐CD107a (LAMP1) antibodies. This flow‐cytometry‐based method allows for the characterization and isolation of serial degranulating NK cells.

## Linked entities

- **Proteins:** LAMP1 (lysosome associated membrane protein 1), LAMP1 (lysosome associated membrane protein 1)

## Full-text entities

- **Genes:** LAMP1 (lysosome associated membrane protein 1) [NCBI Gene 3916] {aka CD107a, LAMPA, LGP120}

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12064871/full.md

## References

12 references — full list in the complete paper: https://tomesphere.com/paper/PMC12064871/full.md

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Source: https://tomesphere.com/paper/PMC12064871