Effect of Celastrus paniculatus on Alcohol-Elicited Conditioned Place Preference and Alcohol Withdrawal-Induced Anxiety in Mice

TL;DR

This study shows that Celastrus paniculatus seed oil reduces alcohol dependence and withdrawal anxiety in mice, similar to existing medications.

Contribution

The study demonstrates the efficacy of Celastrus paniculatus in animal models of alcohol use disorder.

Findings

Celastrus paniculatus reduced alcohol dependence in mice as measured by the CPP model.

The highest dose of CP prevented alcohol withdrawal-induced anxiety in EPM and OFT tests.

CP3 group results were comparable to naltrexone and diazepam in treating alcohol-related behaviors.

Abstract

Background

Celastrus paniculatus (CP), an Indian medicinal plant, is hypothesized to be effective in alcohol use disorder (AUD) based on its medicinal properties useful in CNS disorders as per ancient Ayurveda literature. Hence, it was tested in animal models of AUD.

Methodology

The study was conducted in Swiss albino mice (n=144) of either sex after obtaining Institutional Animal Ethics Committee permission. Three doses of seed oil of CP (CP1: 140, CP2: 280 and CP3: 560 mg/kg) were administered orally in milk (vehicle for CP). A conditioned place preference (CPP) model was used to study the effect of CP on alcohol dependence. Elevated plus maze (EPM) and open field test (OFT) were used to study its effect on alcohol withdrawal-induced anxiety. Naltrexone and diazepam were used as positive controls.

Results

The results in all the CP groups (CP1 (337.88±20.66s, p<0.05); CP2 (322.38±17.61s, p<0.05) and CP3 (315.50±4.24s) as evaluated by the time spent in alcohol paired compartment in CPP) showed better protection against alcohol dependence as compared to the vehicle (552.63±27.47s, p<0.05). The values in all the CP groups were comparable to the naltrexone group. In the EPM test, the CP3 group showed significantly increased time spent (170.63±19.75s) in the open arm (vs the vehicle control, 12.75±11.03s, p<0.05). Also in OFT, longer time was spent in the central zone by mice in the CP3 group (23.25±6.19s vs.8.50±5.48s in the vehicle group, p<0.05). The results of the CP3 group were comparable to diazepam in EPM and OFT experiments.

Conclusion

Celastrus paniculatus seed oil showed a dose-dependent effect in the CPP model of alcohol dependence in mice. The highest dose of CP also prevented alcohol withdrawal-induced anxiety.

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