Characterization of mycobacteria isolated from the Brazilian Atlantic Forest: a public health and bioprospection perspective

TL;DR

This study characterizes mycobacteria from Brazil's Atlantic Forest, exploring their potential impact on public health and biotechnology.

Contribution

The paper provides novel insights into the metabolic and pathogenic potential of previously understudied environmental mycobacteria from the Atlantic Forest.

Findings

The isolates exhibited metabolic activities including cellulase and protease production.

Secreted proteins and shared antigens were profiled, revealing potential ecological and pathogenic roles.

Antigenic similarities to the BCG vaccine strain suggest possible implications for vaccine efficacy.

Abstract

The Mycobacterium genus remains highly relevant today due to the rising incidence of tuberculosis and opportunistic infections caused by environmental mycobacteria. While much is known about M. tuberculosis, M. leprae and M. bovis, studies focusing on environmental mycobacteria remain limited. These microorganisms are globally distributed and have been identified in diverse biomes, including the Atlantic Forest. This study aims to provide a characterization of four mycobacterial strains isolated from the Atlantic Forest, assessing their metabolic capabilities and biotechnological potential. We investigated the presence of cellulases and proteases and conducted an initial profiling of secreted proteins. Furthermore, the examination of shared antigens and infection kinetics within macrophages offered insights into the ecological and pathogenic potential of these isolates. From a public health perspective, antigenic similarities between these environmental microorganisms and the BCG vaccine strain may influence the efficacy of BCG in protecting against diseases such as tuberculosis. Continued research on these and other environmental isolates, particularly within Brazil’s highly biodiverse ecosystems, holds promise for advancing scientific knowledge and contributing to human health.

Figures