Impact of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of young ducks in a rice-duck-crayfish coculture system

TL;DR

This study examines how different stocking densities affect the health and gut microbiota of young ducks in a rice-duck-crayfish coculture system.

Contribution

The study identifies specific changes in duck organ indices, serum biochemistry, and gut microbiota under varying stocking densities in an RDC system.

Findings

High-density stocking increased liver and spleen indices and altered serum biochemistry in ducks.

Increased stocking density led to gut morphology changes and shifts in cecal microbiota composition.

High stocking density reduced beneficial gut bacteria and increased harmful bacteria abundance.

Abstract

The rice-duck-crayfish (RDC) coculture system, an ecologically efficient breeding strategy that accommodates natural behavior of ducks and improves their welfare. The optimal stocking density and its impact on duck health in this system remains undetermined. The study examined the impact of stocking densities on growth, organ index, serum biochemistry, gut morphology and microbiota of ducks in RDC system.

A total of five hundred and forty 20-day-old Nonghu No. 2 ducks were randomly allocated based on density: low-density (LD; 8 birds/666.67 m2), medium-density (MD; 12 birds/666.67 m2) and high-density (HD; 16 birds/666.67 m2) groups, with three replicates in each group, and the symbiosis period was up to 40 days until rice tasselling.

There were no significant differences in final body weight, average daily gain, or feed:gain ratio between groups (p>0.05); however, the liver and spleen indices of ducks in HD group were significantly greater than those in LD group (p<0.05). The serum albumin concentration in HD group decreased, whereas creatine kinase activity increased (p<0.05). Additionally, the ileal crypt depth significantly increased and the ileal villus height and villus/crypt ratio significantly decreased in ducks in MD and HD groups compared to LD group (p<0.05). Moreover, the abundance of cecal Deferribacterota and Spirochaetota increased significantly (p<0.05), while the abundance of Firmicutes decreased significantly (p<0.05) with increasing stocking density. Moreover, the increase in stocking density significantly decreased the abundance of some beneficial bacteria (Faecalibacterium and Fournierella) and increased the abundance of some harmful bacteria (Mucispirillum and Brachyspira) (p<0.05).

These results suggest that moderately HD breeding doesn’t significantly affect duck growth, but increased stocking density led to changes in cecal microbiota and dysbiosis. Reducing stocking density positively affects immune parameters and ileum morphology. However, due to the limited number of total replicates of the study, further research is needed to validate the reliability of the results.

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