Mechanism of total saponins of Ranunculus ternatus Thunb. in treatment of breast cancer based on liquid chromatography–mass spectrometry and network analysis
Yuanxin Zhang, Shuo Nan, Wei Zhao, Haisheng Xie, Jinxin Miao, Mingsan Miao
TL;DR
This study explores how saponins from Ranunculus ternatus may treat breast cancer by affecting specific biological pathways and proteins.
Contribution
The study identifies the JAK2/STAT3 signaling pathway as a potential target of RT saponins in breast cancer treatment.
Findings
RT saponins inhibit JAK2 and STAT3-related proteins and mRNA in breast cancer.
RT saponins reduce tumor growth, proliferation, and migration while promoting apoptosis.
Serum inflammatory factors like TNF-α, IL-6, and IL-10 are affected by RT saponins.
Abstract
To explore the mechanism of total saponins of Ranunculus ternatus Thunb. (RT) in the treatment of breast cancer (BC) using liquid chromatography–mass spectrometry (LC-MS) technology and network analysis. The metabolites of RT were detected using LC-MS. Metabolites and targets of RT and BC were identified in different databases, and potential targets and pathways were predicted using protein–protein interaction network and pathway enrichment analyses. A mouse model of BC created by cellular injection and MCF-7 cells were used as research objects for in vivo and in vitro validation experiments to study the anti-BC mechanism of RT. A Kyoto Encyclopedia of Genes and Genomes analysis showed that the Janus kinase/signal transducer and activator of transcription signaling pathway might be associated with the anti-BC effects of RT. The in vivo and in vitro experiments showed that Total…
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Taxonomy
TopicsChromatography in Natural Products · Pharmacological Effects of Natural Compounds · Metabolomics and Mass Spectrometry Studies