# Molecular cloning and functional characterization of the shikimate kinase gene from Baphicacanthus cusia

## TL;DR

This study identifies and characterizes a new gene in a medicinal plant that helps produce important alkaloids, offering potential for improving its medicinal properties.

## Contribution

The study reports the cloning and functional analysis of a novel shikimate kinase gene from Baphicacanthus cusia.

## Key findings

- The BcSK gene was cloned and shown to encode a functional shikimate kinase enzyme in chloroplasts.
- Overexpression of BcSK in Isatis indigotica increased the production of indole alkaloids like indigo and indirubin.
- BcSK expression was significantly upregulated under abiotic stress and was most active in flowers.

## Abstract

Baphicacanthus cusia (Nee) Bremek, a perennial herbaceous plant with medicinal properties, has limited genomic insights regarding the genes involved in its indole alkaloid biosynthesis pathway. In this study, the BcSK gene was isolated and cloned from the transcriptome data of B. cusia. The full-length cDNA of BcSK is 1,657 bp, comprising a 265 bp 5’ UTR, a 507 bp 3’ UTR, and an 885 bp ORF encoding 295 amino acids. The exon-intron structure of BcSK consists of four exons and three introns. Bioinformatics and phylogenetic analyses revealed a high degree of homology between BcSK and its counterparts in various plant species. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis showed that BcSK expression was significantly altered under abiotic stress conditions, including methyl jasmonate (MeJA), abscisic acid (ABA), and ultraviolet (UV) radiation. The gene was predominantly expressed in flowers compared to roots, stems, and leaves. Subcellular localization analysis indicated that BcSK is primarily expressed in chloroplasts, confirming that the conversion of shikimic acid to shikimate-3-phosphate occurs in this organelle. Prokaryotic expression and enzyme activity assays demonstrated that the heterologously expressed BcSK protein catalyzed the conversion of shikimic acid to shikimate-3-phosphate. Furthermore, the ectopic overexpression of BcSK in Isatis indigotica significantly enhanced the biosynthetic flux toward indole alkaloids, including indole, indigo, and indirubin. In conclusion, this study identifies and characterizes a novel BcSK gene, providing new insights and potential applications for the metabolic engineering of B. cusia.