# Sequence analysis of two F1 mycobacteriophages, Deb65 and DocMcStuffins

**Authors:** Marcus O. Royster, Victoria Figgins, Vera Pande, Jason D. Robinson, Deeka S. Abdi, Ali Amin, Zephaniah Ansah, Ethan W. Bomersheim, Gianna Dunn, Ali A. Elfaki, Jordyn Foulk, Kate C. Ingle, Avi D. Lavu, Ved Pande, Priya T. Shan, Marie P. Smithbey, Gunnar R. Ternstrom, Olivia S. Trager, David A. Washington, Monica Xu, Margaret S. Saha

PMC · DOI: 10.1128/mra.01328-24 · Microbiology Resource Announcements · 2025-04-14

## TL;DR

This paper analyzes the genetic sequences of two mycobacteriophages, Deb65 and DocMcStuffins, isolated from wetland soil and found to infect Mycobacterium smegmatis.

## Contribution

The study identifies 41 shared genes and classifies both phages into the F1 subcluster based on gene content similarity.

## Key findings

- Deb65 and DocMcStuffins encode 97 and 91 putative genes, respectively.
- 41 genes are shared between the two phages.
- Both phages are assigned to the F1 subcluster of actinobacteriophages.

## Abstract

Isolated from wetland soil, Deb65 and DocMcStuffins are bacteriophages with a siphoviral morphology that infect Mycobacterium smegmatis. Deb65 and DocMcStuffins encode 97 and 91 putative genes, 41 of which are shared. Based on gene content similarity to actinobacteriophages more broadly, both phages are assigned to subcluster F1.

## Full-text entities

- **Species:** Mycolicibacterium smegmatis (species) [taxon 1772]

## Full text

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## Figures

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## References

19 references — full list in the complete paper: https://tomesphere.com/paper/PMC12060671/full.md

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Source: https://tomesphere.com/paper/PMC12060671