Regulation of transcription elongation anticipates alternative gene expression strategies across the cell cycle

TL;DR

This study shows how gene expression is controlled during the cell cycle by regulating RNA polymerase activity and mRNA stability.

Contribution

The study identifies distinct transcription elongation control patterns and their connection to ribosome biogenesis genes across the cell cycle.

Findings

Transcription elongation control patterns vary across G1, S, and G2/M phases of the cell cycle.

Genes related to ribosome biogenesis show divergent elongation patterns and increased mRNA expression after G1.

Coordinated regulation of RNA polymerase activity and mRNA decay is essential for gene expression strategies.

Abstract

A growing body of evidence supports the idea that RNA polymerase II (RNAP II) activity during transcription elongation can be regulated to control transcription rates. Using genomic run-on and RNAP II chromatin immunoprecipitation, we measured both active and total RNAP II across the bodies of genes at three different stages of the mitotic cell cycle in Saccharomyces cerevisiae: G1, S, and G2/M. Comparison of active and total RNAP II levels at these stages revealed distinct patterns of transcription elongation control throughout the cell cycle. Previously characterized cycling genes were associated with some of these elongation patterns. A cluster of genes with highly divergent genomic run-on and RNAP II chromatin immunoprecipitation patterns was notably enriched in genes related to ribosome biogenesis and the structural components of the ribosome. We confirmed that the expression of ribosome biogenesis mRNAs increases after G1 but decreases following mitosis. Finally, we analyzed the contribution of mRNA stability to each cluster and found that a coordinated regulation of RNAP II activity and mRNA decay is necessary to fully understand the alternative strategies of gene expression across the cell cycle.

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