# The effects of a plant-based and a plant- and marine-based n-3 oil supplement on behavioral reactivity, heart rate variability, and plasma fatty acid profile in young healthy horses

## TL;DR

This study tested if plant-based and marine-based oils could reduce stress and negative behaviors in young horses, but found no significant effects.

## Contribution

The study is the first to investigate the effects of ALA, EPA, and DHA on heart rate variability and behavior in horses.

## Key findings

- Plasma DHA increased and n-6:n-3 ratio decreased in the algae oil group.
- Neither camelina nor algae oil reduced behavioral reactivity compared to the control.
- Heart rate variability and most behaviors were unaffected by the oil supplements.

## Abstract

Behavioral reactivity in horses poses a welfare and safety risk to both the horse and the handler, however, beneficial effects have been observed when dietary fat is increased in replacement of sugar. Supplementation with the fatty acids (FA) eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) appear to improve negative behaviors in rodents and humans, but the effect of α-linolenic acid (ALA), EPA, and DHA, specifically, on reactivity in horses is unknown. The objective of this study was to evaluate the effects of camelina oil (CAM; ALA-enriched) and a mix of camelina and algal oil (ALG; ALA-, EPA-, and DHA-enriched) both fed at a dose of 0.37 g oil/kg body weight on plasma FA, behavior, and heart rate variability (HRV) in young horses compared to a negative control (CON). Thirty-four client-owned horses aged 7 mo to 6 yr were enrolled. Horses were assigned to either CAM, ALG, or CON and underwent a novel object test (NOT) before and after a 6-wk supplementation period. Prior to each NOT, blood was collected for evaluation of plasma FA profile (n = 28). During the NOT, behavior was recorded using a predetermined ethogram and assessed in BORIS software by 2 raters (n = 29). Electrocardiogram (ECG) data was collected at baseline, during the NOT, and after the NOT (recovery). The ECG data was analyzed in Kubios software for determination of heart rate (HR) and several HRV parameters (n = 24). The treatment oils were treated as fixed effects, baseline measurements as covariates, and location as a random effect. Plasma DHA (P < 0.01) was greater and n-6:n-3 ratio (P < 0.01) was reduced in ALG than in CAM and CON, while ALA and EPA were similar among treatments (P > 0.05). When treatments were pooled, the maximum HR (P < 0.01) and the low frequency to high frequency ratio HRV parameter (P < 0.01) were greater during the NOT compared to baseline and recovery. Bucking (P = 0.03) and backing (P = 0.02) behaviors were reduced in the CAM group compared to the CON group, but neither group differed from ALG. All other behaviors, HR, and HRV parameters were similar among treatments (P > 0.05). Our results suggest that the NOT was successful in creating acute stress, however, feeding either CAM or ALG at this dose did not reduce reactivity in this cohort of horses. Further research is needed to understand the effects of specific FA, if any, on behavior and HRV in more specific populations of horses and specifically those deemed highly reactive.

There is some evidence to suggest that behavioral reactivity in horses can be reduced by increasing fat in the diet. While plasma fatty acids were reflective of the oil consumed, supplementing horses with either camelina oil or a camelina and algae oil mix did not impact heart rate variability or most behavior parameters in horses as compared to a non-supplemented control.