Is there an association between superoxide dismutase gene polymorphisms, antioxidants in oxidative stress pathway, and oral health-related quality of life after root canal treatment?

TL;DR

This study explores how genetic variations in SOD2 and SOD3 genes affect oral health quality of life after root canal treatment.

Contribution

The study identifies specific SOD gene polymorphisms associated with oral health-related quality of life outcomes following dental treatment.

Findings

The SOD3 rs2855262 polymorphism was linked to functional limitation in OHRQoL.

The SOD3 rs13306703 polymorphism significantly affected multiple OHRQoL domains including pain and psychological discomfort.

SOD2 rs5746136 was associated with psychological disability in multivariate analysis.

Abstract

To evaluate the association between genetic polymorphisms in SOD2 and SOD3 and oral health-related quality of life (OHRQoL).

The cohort study included 109 participants, all of whom underwent root canal treatment on a single-rooted maxillary or mandibular tooth with asymptomatic periapical periodontitis. The OHRQoL was assessed using the Oral Health Impact Profile-14 at three intervals: prior to root canal treatment (T0), 7 (T7), and 30 (T30) days post-treatment. Genomic DNA was extracted from buccal cells for genotyping of polymorphisms in SOD2 (rs5746136, rs4880, and rs10370) and SOD3 (rs2855262 and rs13306703) using real-time PCR. Both Univariate and Multivariate Poisson Regression analyses were conducted, with p < 0.05 indicating statistical significance.

The rs2855262 polymorphism in the SOD3 gene showed a significant difference in the functional limitation domain in both codominant (p = 0.037) and recessive (p = 0.040) models. The rs13306703 polymorphism in SOD3 demonstrated a significant difference in physical pain [in codominant (p = 0.001) and recessive (p < 0.001) models], psychological discomfort [in codominant (p = 0.002) and recessive (p = 0.002) models], handicap [in codominant (p = 0.011) and dominant (p = 0.015) models], and total score [in codominant (p = 0.011) and recessive (p = 0.007) models]. In the Multivariate Poisson Regression analysis, SOD2 (rs5746136) was associated with the psychological disability domain [in codominant (p = 0.049) and recessive (p = 0.040) models] and SOD3 (rs13306703), in the handicap domain [in codominant (p = 0.028) and dominant (p = 0.037) models].

Genetic polymorphisms in SOD2 and SOD3 genes can influence the OHRQoL response in patients with asymptomatic periapical periodontitis.

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