Mechanistic Insights Into GDFMD-Mediated Inhibition of Liver Fibrosis via miRNA-29b-3p Upregulation in Wilson's Disease
Peng Huang, Yuzhe Huang, Ting Dong, Han Wang, Meixia Wang, Xiang Li, Wei Dong, Yulong Yang, Wei He, Wenming Yang
TL;DR
This study explores how a traditional Chinese medicine called GDFMD helps reduce liver fibrosis in Wilson's disease by boosting a specific microRNA.
Contribution
The study identifies miRNA-29b-3p upregulation as a novel mechanism through which GDFMD inhibits liver fibrosis in Wilson's disease.
Findings
GDFMD reduces liver fibrosis markers in Wilson's disease patients.
GDFMD increases miRNA-29b-3p levels, which counteracts TGF-β1 effects.
miRNA-29b-3p upregulation by GDFMD inhibits HSC transdifferentiation into myofibroblasts.
Abstract
Background: Wilson's disease (WD) is an abnormal copper metabolism disease. GanDouFuMu decoction (GDFMD) is a traditional Chinese medicine, whicn has shown good therapeutic effects in clinical treatment of WD liver fibrosis;but its regulatory mechanism is still unclear. Methods: The serum of WD patients before and after GDFMD treatment were collected, the four items of liver fibrosis were detected by ELISA. The hepatic stellate cell (HSC) activities were assesed via CCK8 assay. The mRNA levels were evaluated by qPCR. The protein levels were checked by western blot. The autophygosomes were observed by transmission electron microscope (TEM). The transdifferentiation ability of HSCs into myofibroblasts was evaluated with anti-α-SMA antibody by immunofluorescence (IF). In copper-laden rats with WD, the autophagy levels, and fibrosis level were observed by IF. Results: The four items of…
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Taxonomy
TopicsTrace Elements in Health · Liver physiology and pathology · Drug Transport and Resistance Mechanisms