# The effect of the PKR inhibitor, 2-aminopurine, on the replication of influenza A virus, and segment 8 mRNA splicing

## TL;DR

This paper investigates how the PKR inhibitor 2-aminopurine affects influenza A virus replication and the splicing of segment 8 mRNA.

## Contribution

The study explores the novel role of PKR inhibition in regulating influenza A virus mRNA splicing and replication.

## Key findings

- PKR inhibition by 2-aminopurine may influence IAV replication.
- Segment 8 mRNA splicing is regulated and impacts viral proteins NS1 and NS2.
- Further research is needed to clarify PKR's role in IAV mRNA splicing.

## Abstract

Influenza A virus (IAV) is a negative-sense, single-stranded RNA virus, whose genome consists of eight segments encoding multiple structural and non-structural proteins essential for viral replication and host immune evasion. Among these, segment 8 encodes two primary non-structural proteins  NS1, crucial for host immune suppression and virulence and NS2, involved in vRNP export – and their expression is regulated by splicing. Further studies are required to elucidate the role of PKR in IAV mRNA splicing regulation.