Novel endornaviruses infecting Phytophthora cactorum that attenuate vegetative growth, promote sporangia formation and confer hypervirulence to the host oomycete

TL;DR

Two new viruses in a plant pathogen reduce its growth but increase its ability to cause disease and tolerate stress.

Contribution

Discovery of two novel endornaviruses that alter the growth, sporulation, and virulence of Phytophthora cactorum.

Findings

The viruses PcAEV4 and PcAEV5 are associated with hypervirulence and altered stress responses in Phytophthora cactorum.

Virus-free strains showed increased mycelial growth but reduced sporangia formation and virulence.

The viruses localize to the endoplasmic reticulum and affect sterol response in the host.

Abstract

Two novel endornaviruses were found in Phytophthora cactorum isolated from black lesions on Boehmeria nivea var. nipononivea plants in a Japanese forest. These two endornaviruses were named Phytophthora cactorum alphaendornavirus 4 (PcAEV4) and Phytophthora cactorum alphaendornavirus 5 (PcAEV5) and have site-specific nick structures in their positive RNA strands, which are hallmarks of alphaendornaviruses. Ribavirin and cycloheximide treatment of the protoplasts effectively cured the host oomycete (strain Kara1) of the viruses. The resultant virus-free strain (Kara1-C) displayed abundant mycelial growth with less zoosporangia formation as compared to the Kara1 strain. Remarkably, the Kara1-C strain exhibited a reduced ability to form black lesions on B. nivea leaves, suggesting that the presence of PcAEV4 and PcAEV5 in the Kara1 strain led to enhanced virulence in host plants. Under osmotic pressure and cell wall synthesis inhibition, the Kara1 strain exhibited less growth inhibition compared with the Kara1-C strain. In contrast, the Kara1 strain showed more growth inhibition in the presence of membrane-permeable surfactant compared with the Kara1-C strain, indicating that the two endornaviruses can alter the susceptibility of the host oomycete to abiotic stresses. Co-localization and cell fractionation analyses showed that PcAEV4 and PcAEV5 localized to intracellular membranes, particularly the endoplasmic reticulum membrane fraction. Furthermore, infection with these two endornaviruses was found to affect the host’s response to exogenous sterols, which enhanced vegetative growth and zoosporangia formation, as well as virulence of the host oomycete. These results provide insights into the effects of endornavirus infection in Phytophthora spp. and also highlight the usefulness of protoplast-based methods in advancing Phytophthora virus studies.

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